Table 3. Selection of BRAF mutant alleles in the progression of melanoma.
|
Subcloning (no. of colonies)
|
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|---|---|---|---|---|---|---|---|---|
| Sample no. | Age (years) | Sex | Type of melanoma | Site | Tissue | Direct sequencinga | BRAF mutationsa | Wild type |
| PM-10 | 32 | F | NCSD | Forearm | FFPE | Wild type | 2(V600E) | 38 |
| RPM-10 | Forearm | FFPE | V600E | 7(V600E) | 33 | |||
| PM-16 | 53 | F | NCSD | Neck | Frozen | V600−K601E | 9(V600−K601E)+2(V600E) | 30 |
| MM-16 | Cervical LN | Frozen | V600−K601E | 27(V600-K601E) | 13 | |||
| PM-17 | 67 | M | NCSD | Right shoulder | Frozen | Wild type | 8(V600K)+1(K601E) | 71 |
| MM-17-1 | Skin | Frozen | V600K | 13(V600K) +1(K601E) +1(K601K) | 15 | |||
| MM-17-2 | Right lung | Frozen | Wild type | NE | NE | |||
| MM-17-3 | Small intestine | Frozen | V600K | 14(V600K) | 26 | |||
| MM-17-4 | Right chest wall | Frozen | V600K | 15(V600K)+1(K601E) | 54 | |||
| MM-17-5 | Pulmonary LN | Frozen | Wild type | NE | NE | |||
| MM-17-6 | Supraclavicular LN | Frozen | V600K | 9(V600K)+1(K601E) | 30 | |||
| MM-17-7 | Infraclavicular LN | Frozen | V600K | 22(V600K) | 17 | |||
| MM-17-8 | Axillary LN | Frozen | V600K | 12(V600K) | 28 | |||
| MM-17-9 | Diaphragm | Frozen | V600K | 11(V600K) | 28 | |||
FFPE=formalin-fixed, paraffin-embedded tissue; LN=lymph node; MM=metastasis; NCSD=melanoma on non-chronic sun-damaged skin; NE=not examined; PM=primary melanoma; RPM=recurrent primary melanoma.
a
V600E, T1799A; V600-K601E, TGA1799-1801del; V600K, GT1798-99AA; K601E, A1801G.