Table 2.

Applied RNAi control experiments - overview

Control experiment	Type	Example
Positive control siRNA	siRNA that is known to provide high knockdown of its target gene	Hs_MAPK1 siRNA (Qiagen) OrderNo. 1022564
Negative control siRNA	a nonsilencing siRNA with no homology to any known mammalian gene	AllStars Negative control siRNA (Qiagen) OrderNo. 1027280
Transfection control siRNA	siRNA that is used to measure the transfection efficiency, e.g. by siRNA	AllStars Hs Cell Death Control siRNA (Qiagen) OrderNo. 1027298
Mock transfection control	control experiment where cells go through the transfection process without addition of siRNA
Untransfected cells control	control experiment where gene expression analysis is carried out on cells that have not gone through the transfection process