Figure 4. Unlinkase activity remains unchanged during poliovirus infection.

HeLa cells were infected with wild type poliovirus and cytoplasmic extracts were collected at 0 (lanes 3 and 9), 2 (lanes 4 and 10), 4 (lanes 5 and 11), or 6 (lanes 6 and 12) hours post-infection. 1 µg (lanes 1–6) or 5 µg (lanes 7–12) of total protein from these extracts was incubated with full-length ³⁵S-methionine radiolabeled W1-VPg 31 virion RNA substrate. No difference in activity was observed between the mock lanes (lanes 1, 2, 7, and 8) and the different time points collected during the infection.