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. 2011 Mar 7;6(3):e16559. doi: 10.1371/journal.pone.0016559

Figure 7. Unlinkase activity on a poliovirus versus a rhinovirus substrate.

Figure 7

(A) VPg sequences of HRV14 and S1-VPgR1 (wild type poliovirus mutated to encode an HRV14 VPg that encodes two methionine residues). (B) Single-cycle growth assay of wild type poliovirus (closed circles) and S1-VPgR1 (closed squares). 35S-methionine radiolabeled W1-VPg 31 or S1-VPgR1 substrate that was treated with RNase T1 (C) or untreated (D) was incubated with increasing amounts of total protein from a partially-purified fraction of unlinkase activity (Fraction CA) ((C) 0, 5, 10, and 20 µg and (D) 0, 0.5, 1, 2, 5 and 10 µg) to demonstrate that unlinkase activity recognizes each substrate equally.