Table 2. Comparison of the percent homologies (%) of the complete HSFY protein (A) and HSF-type DNA-binding domain (B), for all species analyzed.
human (hHSFY2) | mouse (mHSFYL) | rat | rhesus macaque | cat | bovine | |||||||
A | B | A | B | A | B | A | B | A | B | A | B | |
human (hHSFY1) | 100 | 100 | 43 | 71 | 44 | 71 | 81 | 87 | 64 | 78 | 51 | 72 |
human (hHSFY2) | - | - | 43 | 71 | 44 | 71 | 81 | 87 | 64 | 78 | 51 | 72 |
mouse (mHSFYL) | - | - | 95 | 100 | 43 | 69 | 47 | 80 | 40 | 76 | ||
rat | - | - | 43 | 69 | 46 | 80 | 39 | 76 | ||||
rhesus macaque | - | - | 58 | 73 | 43 | 69 | ||||||
cat | - | - | 58 | 81 |
In all cases, the percent homology was highest at the conserved HSF-type DNA-binding domain with the exception of an analysis of the two human HSFY copies which showed that both the complete protein sequence and conserved region were identical (100% homology). Bovine showed 51% and 72% homology with human HSFY for the complete protein and conserved domain, respectively. Bovine HSFY was most closely related to cat HSFY with a complete protein homology and conserved domain homology of 58% and 81%, respectively. The accession numbers for the proteins found in the NCBI database are as follows: human hHSFY1: NP_149099.2; hHSFY2: NP_714927.1, mouse NP_081937.1, rat NP_001012132.1, rhesus macaque ACL51668.1, cat NP_001035212.1, bovine NP_001070474.1.