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. Author manuscript; available in PMC: 2011 Mar 7.
Published in final edited form as: Dev Biol. 2006 Jul 8;298(2):498–513. doi: 10.1016/j.ydbio.2006.06.054

Fig. 2.

Fig. 2

Fndc3a is deleted in sys mice and is expressed in wild-type adult male testis. (A) Southern blot analysis of genomic DNA from wild-type and sys mice using a Fndc3a mouse cDNA as a probe. The hybridization signals for Fndc3a are absent in lanes containing genomic DNA from sys homozygous mutants (−/−). (B) Northern blot analysis of total RNA from testes of wild-type, heterozygous, and homozygous sys mice. Left panel; gel was stained with ethidium bromide. Right panel; blot hybridized with a cDNA probe representing nucleotides 4869–6139 of Fndc3a. This probe detects an Fndc3a-specific transcript of approximately 6 kb in size in testis RNA from wild-type and heterozygous sys mice but no transcript in testicular RNA from homozygous sys mutants.