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. Author manuscript; available in PMC: 2011 Mar 7.
Published in final edited form as: Dev Biol. 2006 Jul 8;298(2):498–513. doi: 10.1016/j.ydbio.2006.06.054

Fig. 8.

Fig. 8

Western analysis of FNDC3A expression in enriched preparations of germ cells. Total protein extracts (15 μg, except 25 μg sample from homozygous mutant) were subjected to SDS-PAGE followed by blotting, and incubated with FNDC3A antisera. Key: RB, Residual bodies; Pa1, low enrichment pachytene spermatocytes; Pa2, highly enriched pachytene spermatocytes; RS, round spermatids; Te +/+, whole testis from wild-type mouse; Te −/−, whole testis from Fndc3asys homozygous mouse. FNDC3A (arrow) is enriched in round spermatids compared to the other germ cell enriched-fractions and whole testis. The nonspecific band at approximately 52 kDa was used to monitor the relative amount of protein loaded per lane. Due to the reduced amount of protein loaded, the relatively high molecular weight band (arrowhead) observed in the testis extract from the homozygous mutant and preparations of enriched germ cells appears faint in the testis extract from the wild-type animal.