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. Author manuscript; available in PMC: 2011 Mar 7.
Published in final edited form as: Nature. 2004 Jan 29;427(6973):461–465. doi: 10.1038/nature02229

Figure 1.

Figure 1

Preparation of a CRE-conditional Ant2-null mutant allele in mouse embryonic stem cells. a, Top to bottom, maps of the wild-type Ant2 locus, the region of homology within the targeting construct, and the floxed (Ant2fl) and mutant (Ant2–) alleles. Exons (black boxes), loxP sites flanking exons 3 and 4 (triangles), PGKneo selection cassette within 3′UTR (hatched box). a, b, Arrows indicate sizes of Xba I (X) and Dra III (D) restriction fragments used to identify correctly targeted clones by Southern analysis of embryonic-stem-cell genomic DNA using probes A and B. b, Southern blot of targeted and untargeted embryonic-stem-cell clones.