Fig. 5. PCR based analysis of gene expression in PGCs and somatic tissues from mouse embryos.
(A) Expression of M 96, Dlg2-like, fsh-related and Hprt (control) was determined by PCR analysis of the following cDNA samples-(M)-marker; (1) e6.5 epiblast; (2) e11.5 PGC; (3) e12.5 PGC; (4) e13.5 PGC; (5) e13.5 male PGC; (6) e13.5 female PGC; (7) e12.5 F(−) somatic cells. (B) Expression of fsh-related gene (H0503B12) during embryonic development and in adult mouse tissues. Northern blots containing adult (upper panel) or embryonic mouse total RNA (lower panel) were hybridized with a fsh-related cDNA. (1) heart; (2) brain; (3) spleen; (4) lung; (5) liver; (6) muscle; (7) kidney; (8) testis; (9) e7.5; (10) e11.5; (11) e15.5; (12) e17.5. Molecular weight sizes are in kb. (data courtesy of Rhonda H. Nicholson). (C) H0503B12 (fsh-related) maps to mouse chromosome 17 within the t-complex. fsh-related was mapped using an inter-specific species backcross panel (Rowe et al., 1994). (Data courtesy of Xueqian Wang).