Protein expression of Glut-4, GSK-3β and mTOR in myocardium from low fat (LF) and high fat (HF)-fed WT and AMPK KD mice. A: Total Glut-4; B: Membrane fraction of Glut-4; C: GSK-3β; D: mTOR; E: phosphorylated mTOR (pmTOR) and F: pmTOR/mTOR ratio. Inset: Representative gel blots depicting total Glut-4, membrane GLUT4, GSK-3β, mTOR and pmTOR. Mean ± SEM, n = 4-5 per group, * p <0.05 vs. WT-LF group, # p < 0.05 vs. WT-HF group.