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. Author manuscript; available in PMC: 2012 Apr 1.

Published in final edited form as: Immunobiology. 2010 Sep 24;216(4):447–456. doi: 10.1016/j.imbio.2010.09.008

Immature monocyte derived DCs were stimulated for 48 hours with 10ug insulin + 10ug CTB, 20ug CTB-INS and PMA + Ionomycin as a positive control for DC activation. The activation and maturation of DCs measured by expression of CD86 and CD83 cell surface markers was determined by flow cytometric methods. The mean fluorescence intensity (MFI) and percentage population data is representative of four repeated independent experiments with comparable results. (Panel A) The overlapping histograms depict the expression of CD83 and CD86 costimulatory factors in immature dendritic cells after stimulation with PMA + Ionomycin, Insulin + CTB and CTB-INS. The statistical significance was calculated based on P<0.005.

Immature monocyte derived DCs were stimulated for 48 hours with 10ug insulin + 10ug CTB, 20ug CTB-INS and PMA + Ionomycin as a positive control for DC activation. The activation and maturation of DCs measured by expression of CD86 and CD83 cell surface markers was determined by flow cytometric methods. The mean fluorescence intensity (MFI) and percentage population data is representative of four repeated independent experiments with comparable results. (Panel A) The overlapping histograms depict the expression of CD83 and CD86 costimulatory factors in immature dendritic cells after stimulation with PMA + Ionomycin, Insulin + CTB and CTB-INS. The statistical significance was calculated based on P<0.005.