Figure 3.

Rin1 is required for transformation of primary bone marrow (BM) cells to growth factor independence. (a) BM cells from wild-type or Rin1^−/− mice were infected with a BCR-ABL1 (p210) retrovirus, cultured without growth factors and counted at indicated times. (b) BM cell transformation as in ‘a', except using ETV6-ABL1 (a.k.a. TEL-ABL) retrovirus. (c) BM cell transformation as in ‘a', except using virus expressing both BCR-ABL1 and RIN1^ABD. (d) BM cell transformation as in ‘c', except using a mutant (RIN1^ABDmut) that does not bind ABL1. (e) BM cell transformation as in ‘a', except using v-Abl retrovirus. (f) BM cell transformation as in ‘a', except using the multidrug-resistant mutant BCR-ABL1^T315I. All results are for duplicate samples counted in triplicate. Bars show standard deviation; ^* indicates P<0.05 between wt and Rin1^−/−. Panel f shows 14-day sample P=0.06 between wt and Rin1^−/−.