Skip to main content
. 2010 Nov 19;25(2):290–300. doi: 10.1038/leu.2010.268

Figure 4.

Figure 4

Analysis of human leukemia cells. (a) Panel of CML and ALL cell lines immunoblotted with anti-ABL1 (top), anti-RIN1 (middle) or anti-TUBB (bottom). CML cells (1=KCL22; 2=JURL-MK1; 3=K562) and a B lymphoid CML blast crisis cell line (4=BV173) express the p210 form of BCR-ABL, whereas ALL-derived cells (5=TOM-1; 6=SUP-B15) express the p190 form (arrowheads). Full-length RIN1 is marked with an arrow (faster migrating bands may be alternately spliced isoforms). (b) TOM-1, KCL22, K562 and primary pre-B-ALL cells infected with control (ctr) or RIN1-directed (kd) shRNA were analyzed by immunoblot with anti-phosphotyrosine. MW (kDa) markers are at left, arrowheads mark bands most clearly reduced by RIN1 silencing. Lower panels show immunoblots for TUBB, RIN1 and BCR-ABL1.