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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Arthropod Struct Dev. 2011 Feb 19;40(5):395–408. doi: 10.1016/j.asd.2010.10.004

Figure 1. Mushroom bodies of adult Manduca sexta.

Figure 1

A-C. Anti-DC0 (magenta) and phalloidin (green) labeling of the mushroom body in one hemisphere of the protocerebrum in a twelve day old adult. Phalloidin labels an ingrowth core (arrowheads) made up of the axons of newborn Kenyon cells, which are generated by one neuroblast at the center of each calyx (large arrows). Small arrow in A indicates dense neuropil island in the pedunculus that forms in the pupal stage and is innervated by protocerebral neurons (Figure 6). D. Anti-DC0 (magenta) and anti-BrdU labeling (green) confirms mitotic activity of the mushroom body neuroblasts (arrows) in a six day old adult. E. Fluorescent dextran labeling of the Y-tract (Y), dorsal (d) and ventral (v) lobelets in the pharate adult. F-G. Anti-DC0 (magenta) and phalloidin (green) labeling of outgrowing Class III Kenyon cell axons (arrowheads) in the Y tract, emerging from two clusters of Class III Kenyon cell somata (G) in a twelve day old adult. H-I. Anti-DC0 (magenta) and anti-BrdU (green) labeling confirms mitotic activity of two proliferating neuroblasts amidst the Class III Kenyon cell soma (arrowheads), outside of the mushroom body calyces (H = pharate adult, I = six day old adult). Small arrow in I indicates neuropil island in the pedunculus. J-K. Fluorescent dextran labeling of inputs to the calyces from the antennal lobes (green) and the gustatory center of the subesophageal ganglion (magenta) in a pharate adult. Inner antennocerebral tract (iACT) projection neurons from the antennal lobe innervate most of the calyx neuropil (J, arrowheads) while tritocerebral tract (TT) projection neurons from the subesophageal ganglion innervate a ventroposterior putative accessory calyx neuropil (K, arrowheads). A dorsal compartment of the calyces characterized by small, diffuse microglomeruli was not innervated by neurons from either source (J-K, arrows). Ca- calyx, M- medial lobe, Pe- pedunculus, V- vertical lobe, *- accessory calyx. Scale bars = 50μm for all panels.