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. Author manuscript; available in PMC: 2011 Mar 8.
Published in final edited form as: Gut. 2009 Nov 18;59(2):170–180. doi: 10.1136/gut.2009.188375

Figure 2. NOX5-S expression, H2O2 production and cell proliferation after TDCA treatment in FLO OA cells.

Figure 2

(A) 10−11M taurodeoxycholic acid (TDCA, 24h) significantly increased 3H-thymidine incorporation. (B) Western blot analysis, (C) summarized data and (D) H2O2 measurement showed that 10−11 M TDCA significantly increased NOX5-S expression and H2O2 production. (E) Knockdown of NOX5-S by NOX5 small interfering RNA blocked TDCA-induced increase in thymidine incorporation. The data suggest that TDCA may increase NOX5-S expression, H2O2 production and cell proliferation in FLO cells and that TDCA-induced increase in cell proliferation may depend on activation of NOX5-S. N=3, ANOVA, * P<0.05, ** P<0.01, compared with control.