Thin frozen-thawed brain sections were incubated at room temperature for 90 min in the absence of calcium (Control), in the presence of 2.0 mM calcium (Ca++), and in the presence of 10 μM calpain inhibitor III (CI). They were then processed for immunohistochemistry with anti-stargazin antibodies.
A. Representative images of brain sections incubated under each condition.
B & C. Quantitative analysis of images similar to those shown in A. Staining levels were analyzed in cerebellar molecular layer (B) and granule layer (C). Results were expressed as percent of the values found under control conditions and are means ± S.E.M. of 12 sections. *p<0.001.