After calpain and calcium treatment, samples were processed for western blot analysis of TARP γ-8 as described under Figure 6 and detailed in Materials and Methods. Right arrow points to the doublet band labeled with the TARP γ-8 antibody with an apparent molecular weight of 43–45 kD.
Top: representative image of a western blot under each condition.
Bottom: quantitative analysis of western blots similar to those shown on top. Results were expressed as percent of the values found under control conditions and are means ± S.E.M. of 3 experiments.