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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Photochem Photobiol. 2010 Dec 8;87(2):387–398. doi: 10.1111/j.1751-1097.2010.00859.x

Figure 7.

Figure 7

(A) Injection of the UV-2240 tumor cell line into WT, CD4−/− and CD8−/− mice (a & b). Tumor growth was monitored till 3 weeks. The tumor in CD8−/− mice grew continuously without regression (*, p<0.05., #, p<0.01). (B) (a) Cytokines analysis (IL-17, IFN-γ, IL-10 and IL-4) of CD4+ T-cells from WT and CD8−/− mice after 3 weeks of UV-2240 injection. Cytokines analysis (b) (IL-17, IL-10 and IL-4) (c) IFN-γ of CD8+ T-cells from WT and CD4−/− mice after 3 weeks of UV-2240 injection. Purified T-cell suspensions were prepared from spleen and lymph nodes and were then re-stimulated in culture for 48 hours in anti-CD3 coated plates. (*, p<0.05) There were five mice per group and results are expressed as mean ± SD. T (C) To check the specificity of T-cells against 2240 tumor cells, WT mice were injected with the cell line subcutaneously and 10 days later CD4+ and CD8+ T-cells were isolated from draining lymph nodes. CD11c+ cells were isolated from spleens of tumor bearing and naïve mice and later co-cultured with CD4+ and CD8+ T-cells from tumor bearing mice for 72h and (a) IFN-γ or (b) IL-17 levels detected by ELISA.