A. Microdissected hippocampal homogenates were isolated from P9, 3-week, and 6-week-old WT mice and 6-week-old AQP4-/- mice and subjected to Western blot analysis for AQP4 and actin. Representative immunoblots demonstrate developmental regulation of AQP4 hippocampal protein levels (lower band, AQP4; upper band, actin loading control).
B. Densitometric analysis demonstrates developmental upregulation of AQP4 expression from P9, 3-week, and 6-week samples (expressed as % actin expression) (n=3 per time point). *, p<0.05 compared to 6 weeks; **, p<0.01 compared to P9.