Figure 8. Co-expression of AQP4 and K_ir4.1 mRNA by identified hippocampal glial cells.

A. A freshly isolated astrocyte was de- and hyperpolarized between +30 mV and -120 mV (10 mV increments; holding potential -60 mV; left). Then, the same protocol was applied after adding 100 μM BaCl₂ to the bath (middle); the right panel shows the Ba²⁺-sensitive current.

B. I/V-relation of the Ba²⁺-sensitive currents (left). Note that currents reversed close to the K⁺ equilibrium potential (-82.3 mV). DNA gels demonstrate that the recorded cell co-expressed K_ir4.1 and AQP4 transcripts (right).

C. An NG2 cell was analyzed as described above (holding potential -70 mV; de- and hyperpolarization between +20 and -130 mV) (left). The cell also co-expressed AQP4 and K_ir4.1 mRNA (right).