TABLE 1.
Gene | Sequence | Position | Product length | GeneBank Accession No. |
---|---|---|---|---|
Kir4.1 | se 5‘-TATCAGAGCAGCCACTTCACCTTC | -32 | 570 bp | NM_020269 |
as 5‘-GGATCGTCTCGGCCCTCTTCTTAG | 515 | |||
Kir4.1 (nested) | se 5‘-TTCACCTTCGAGCCAAGATGACG | -11 | 338 bp | |
as 5’-AGGCGTGTGGTTGGCAGGAG | 302 | |||
AQP4 | se 5‘-AGCCGGCATCCTCTACCTG | 387 | 427 bp | NM_009700 |
as 5’-CTGCGCGGCTTTGCTGAA | 796 | |||
AQP4 (nested) | se 5‘-CTGGCCATGGGCTCCTGGTG | 467 | 320 bp | |
as 5’-AAGGCGACGTTTGAGCTCCACATC | 763 |
‘Se’ and ‘as’ mark sense and antisense primers. Position 1 is the first nucleotide of the initiation codon. Kir4.1 and AQP4 sense and antisense primers are located on different exons, respectively.