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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Cancer Res. 2011 Jan 14;71(5):1904–1911. doi: 10.1158/0008-5472.CAN-10-3430

Figure 1. SG treatment results in decreased global cytosine methylation and increased expression of epigenetically silenced genes in human cells.

Figure 1

(A) The percentages of global cytosine methylation in genomic DNA isolated from Jurkat-T cells that were either treated with SG alone or pretreated with MG132 (25 μM) for 2 h and then treated with SG (3 μM) for the indicated time periods. (B) The percentages of global cytosine methylation in genomic DNA isolated from HEK-293T, HL-60 and CEM cells that were untreated or treated with SG (3 μM) for the time periods indicated. (C) Change in mRNA expression in Jurkat-T cells following treatment with SG for 24 h (white bar) or 48 h (black bar). The data represent the means and standard deviations of results from three independent drug treatments. The paired t-test was performed to evaluate the difference between control samples and treated samples in (A) and (B) (*, P < 0.05; **, P < 0.01; ***, P < 0.001).