Table 2.
Differential expression of plasma membrane proteins in logarithmic (Log) versus metacyclic (Meta) promastigotes of L. chagasi.
| FUNCTION | ACCESSION | Da | pI | TMD | Log (AA%) |
Meta (AA%) |
Peptides found |
|---|---|---|---|---|---|---|---|
| ADP,ATP carrier protein 1, mitochondrial precursor |
A4H9X7 | 35545.00 | 10.05 | 5 | 4.98 | 9.35 | 2 |
| Alpha tubulin | A4H727 | 53521.40 | 5.03 | 1 | 6.39 | 10.10 | 4 |
| Beta tubulin | P21148 | 50020.80 | 4.57 | 2 | 7.19 | 6.53 | 3 |
| Enolase | A4HW62 | 46008.10 | 5.20 | 1 | 7.46 | 3.77 | 2 |
| Gim5A protein, putative; glycosomal membrane |
A4HN41 | 24812.8 | 8.51 | 3 | 11.56 | 12.44 | 2 |
| Histone H4 | A4HSP4 | 11418.20 | 10.89 | 0 | 18.00 | 18.00 | 2 |
| Kinesin | A4I0A6 | 75199.00 | 9.84 | 1 | 5.03 | 3.81 | 3 |
| Kinetoplastid membrane protein 11B |
Q25297 | 11170.40 | 6.25 | 0 | 46.74 | 27.17 | 5 |
| Lanosterol 14-alpha- demethylase |
A2TEF2 | 54119.70 | 7.62 | 3 | 6.46 | 6.46 | 2 |
| MSP# | P15706 | 63808.60 | 6.83 | 4 | 5.34 | 6.84 | 4 |
| Paraflagellar rod component |
A4HU55 | 68098.33 | 5.09 | 1 | 4.79 | 7.60 | 4 |
| Paraflagellar rod protein 1D |
A4HIY0 | 69219.20 | 5.29 | 0 | 17.48 | 21.01 | 15 |
| Paraflagellar rod protein 2C |
A4HX39 | 68860.50 | 5.28 | 0 | 8.51 | 15.53 | 9 |
| Paraflagellar rod protein 2C |
A4H8S1 | 68544.30 | 5.35 | 0 | 8.85 | 9.02 | 5 |
| Putative | A4H8W1 | 36235.20 | 5.87 | 1 | 8.83 | 6.94 | 2 |
| Putative | A4HYD4 | 41395.10 | 4.88 | 1 | 9.70 | 9.43 | 3 |
| Putative | A4HZV8 | 49801.80 | 5.49 | 0 | 22.78 | 5.69 | 8 |
| Putative | A4I0B5 | 62548.10 | 6.59 | 3 | 14.21 | 8.45 | 7 |
| Putative | A4I0F1 | 14558.30 | 4.07 | 0 | 24.43 | 24.43 | 2 |
| Putative | A4IDT7 | 71114.90 | 5.63 | 1 | 7.52 | 5.76 | 5 |
| Soluble n-ethylmaleimide sensitive factor |
A4HYZ8 | 32034.60 | 4.99 | 0 | 14.89 | 9.57 | 4 |
| Ubiquitin | Q05550 | 8544.60 | 7.58 | 0 | 32.89 | 44.74 | 2 |
| Vacuolar ATPase subunit- like protein |
A4H4F9 | 41620.60 | 4.89 | 0 | 13.45 | 7.00 | 4 |
| Vacuolar ATPase subunit- like protein |
A4HSN6 | 41528.50 | 4.70 | 0 | 13.45 | 5.04 | 4 |
| 19S proteasome regulatory subunit |
A4HBS3 | 54334.20 | 6.01 | 0 | 5.49 | 2 | |
| 22 kDa potentially aggravating protein papLe22 |
Q9NJS2 | 22098.40 | 9.32 | 3 | 6.60 | 2 | |
| Acyl carrier protein | A4HFE7 | 16705.60 | 5.93 | 0 | 21.33 | 2 | |
| ATP-dependent RNA helicase |
A4HMX7 | 94335.70 | 6.13 | 1 | 5.47 | 4 | |
| Calmodulin | Q25420 | 15481.50 | 4.09 | 0 | 24.29 | 2 | |
| Cytochrome b5-like | A4H5X6 | 13146.60 | 4.70 | 1 | 24.79 | 2 | |
| Cytochrome C oxidase subunit VI |
A4HZH7 | 19241.30 | 7.96 | 0 | 19.75 | 2 | |
| DNAJ domain protein | A4I0P2 | 88800.40 | 5.93 | 4 | 4.16 | 2 | |
| Dynein arm light chain | A4I0S3 | 27254.10 | 5.63 | 0 | 10.13 | 2 | |
| Eukaryotic translation initiation factor |
A4HGT5 | 46318.30 | 4.92 | 1 | 21.48 | 6 | |
| Gim5A protein | A4IBQ9 | 24783.70 | 8.63 | 3 | 12.44 | 2 | |
| Heat shock protein | Q4QDQ2 | 91746.30 | 5.02 | 1 | 3.52 | 2 | |
| Heat shock protein 83 | P27890 | 52658.60 | 5.54 | 0 | 7.08 | 3 | |
| Heat shock protein 83-1 | A4HL69 | 41393.60 | 4.80 | 0 | 6.96 | 2 | |
| Heat shock protein 83 | P27741 | 80534.30 | 4.85 | 1 | 5.28 | 3 | |
| Long chain fatty Acyl CoA synthetase |
A4HRT4 | 78855.60 | 7.63 | 3 | 3.84 | 2 | |
| Methionyl-tRNA synthetase |
A4HZ82 | 84145.70 | 5.63 | 1 | 3.61 | 2 | |
| n-Acyl-L-amino acid amidohydrolase |
A4HZ04 | 42737.60 | 5.02 | 2 | 7.34 | 2 | |
| Phosphogluconate dehydrogenase |
Q18L02 | 51912.10 | 5.74 | 4 | 6.89 | 2 | |
| Poly(a)-binding protein | A4HDV5 | 62023.60 | 9.42 | 0 | 3.62 | 2 | |
| Probable citrate synthase | A4HXU4 | 52159.60 | 7.89 | 2 | 7.02 | 2 | |
| Probable proton ATPase 1A |
P11718 | 107410.60 | 5.29 | 9 | 6.98 | 6 | |
| Proteasome activator protein pa26 |
A4IAX3 | 23945.40 | 5.23 | 0 | 18.39 | 3 | |
| Proteasome alpha 7 subunit |
A4H9T4 | 34861.80 | 7.39 | 0 | 7.14 | 2 | |
| Pteridine transporter ft3 | A4HUE7 | 76188.40 | 6.10 | 14 | 4.43 | 2 | |
| Pteridine transporter ft6 | A4HUE5 | 76005.20 | 5.63 | 14 | 1.88 | 2 | |
| Putative | A4H5E9 | 39862.50 | 6.41 | 0 | 7.02 | 3 | |
| Putative | A4H6H3 | 53945.60 | 8.43 | 4 | 6.22 | 3 | |
| Putative | A4H9P7 | 26979.60 | 7.33 | 0 | 10.64 | 2 | |
| Putative | A4HKK8 | 26364.00 | 5.46 | 0 | 11.64 | 2 | |
| Putative | A4HM53 | 14448.20 | 10.06 | 1 | 26.89 | 4 | |
| Putative | A4HQC5 | 84125.60 | 5.57 | 2 | 2.92 | 2 | |
| Putative | A4HTP3 | 42020.60 | 7.28 | 1 | 6.30 | 2 | |
| Putative | A4HTX5 | 93405.40 | 8.82 | 4 | 2.83 | 2 | |
| Putative | A4HWV0 | 36798.80 | 7.02 | 0 | 7.93 | 2 | |
| Putative | A4HXA8 | 36292.30 | 5.64 | 1 | 17.03 | 5 | |
| Putative | A4HZ55 | 11111.80 | 4.81 | 1 | 11.46 | 2 | |
| Putative | A4I0T3 | 13296.80 | 6.52 | 1 | 19.13 | 2 | |
| Putative | A4I1A0 | 11161.60 | 9.20 | 0 | 24.24 | 2 | |
| Putative | A4I1Q2 | 43111.40 | 6.44 | 3 | 9.14 | 2 | |
| Putative | A4I465 | 46343.40 | 5.84 | 2 | 15.88 | 5 | |
| Putative | A4I535 | 56359.60 | 4.82 | 0 | 36.48 | 12 | |
| Putative | A4I551 | 56465.1.0 | 8.08 | 0 | 5.34 | 2 | |
| Putative | A4I683 | 36279.50 | 6.32 | 5 | 7.78 | 2 | |
| Putative | A4I6L1 | 93786.00 | 8.92 | 6 | 2.38 | 2 | |
| Putative | A4I8B0 | 32807.20 | 4.92 | 1 | 11.00 | 2 | |
| Putative | A4IAY6 | 17953.00 | 7.24 | 0 | 16.97 | 2 | |
| Putative | A4IDM5 | 17414.60 | 8.55 | 0 | 24.38 | 3 | |
| Putative | A4IDN1 | 114046.50 | 7.16 | 7 | 2.18 | 2 | |
| Putative | A4IDT2 | 103400.70 | 6.83 | 4 | 3.01 | 2 | |
| Radial spoke protein 3 | A4HFH8 | 42091.30 | 6.62 | 0 | 7.84 | 2 | |
| Ribonucleoprotein p18, mitochondrial precursor |
A4HWC9 | 21275.50 | 6.93 | 0 | 16.58 | 3 | |
| Ribonucleoprotein p18, mitochondrial precursor |
A4HWD0 | 21305.50 | 6.93 | 0 | 8.56 | 2 | |
| Stress-induced protein sti1 | A4H5F0 | 62414.10 | 6.11 | 0 | 6.22 | 3 | |
| Stress-induced protein sti1 | A4HTP4 | 62201.10 | 5.84 | 0 | 5.13 | 2 | |
| Translation elongation factor 1-beta |
A4I9P1 | 25920.50 | 5.00 | 0 | 12.13 | 2 | |
| Vacuolar-type proton translocating pyrophosphatase 1 |
A4HJA5 | 83592.20 | 4.86 | 16 | 5.36 | 3 | |
| Zinc transporter-like protein |
A4I745 | 48193.60 | 5.92 | 7 | 5.10 | 2 | |
| Adenylate nucleotide translocase |
A7LBL5 | 36215.2 | 9.82 | 5 | 5.52 | 2 | |
| Calpain-like cysteine peptidase |
A4HYX4 | 14987.30 | 5.18 | 0 | 16.03 | 2 | |
| CPC cysteine peptidase | A4I4D6 | 36995.8 | 5.10 | 3 | 20.00 | 5 | |
| Cysteine protease | Q8WT30 | 37009.8 | 5.10 | 3 | 15.88 | 4 | |
| Cytochrome C oxidase subunit VI |
A4HD57 | 22258.30 | 6.24 | 0 | 14.80 | 2 | |
| Cytoskeleton-associated protein CAP5.5 |
A4I6E4 | 79917.3 | 5.39 | 1 | 7.87 | 4 | |
| Dehydrogenase-like protein |
A4HUB6 | 44901.5 | 8.38 | 3 | 6.55 | 2 | |
| Enolase | A4H7T5 | 53692.20 | 7.89 | 3 | 6.81 | 2 | |
| Flagellar glycoprotein-like protein |
A4HUH5 | 62330.0 | 7.66 | 5 | 6.24 | 3 | |
| Fructose-1,6-bisphosphate aldolase |
A4ICK8 | 40694.6 | 8.68 | 1 | 7.01 | 2 | |
| Glycosomal membrane protein |
A4I3V7 | 23759.8 | 10.25 | 2 | 13.96 | 2 | |
| Membrane-bound acid phosphatase precursor |
A4ICG3 | 57288.5 | 7.67 | 3 | 5.45 | 3 | |
| MSP# | A4HUF6 | 63517.4 | 6.96 | 5 | 7.02 | 3 | |
| MSP# | P23223 | 62911.0 | 6.42 | 4 | 4.24 | 2 | |
| MSP# | Q27673 | 63013.1 | 6.91 | 2 | 2.51 | 2 | |
| MSP | A4I3D1 | 60546.0 | 7.41 | 4 | 15.34 | 5 | |
| Paraflagellar rod protein 1D |
A4I4N5 | 69017.00 | 5.17 | 0 | 5.71 | 4 | |
| Proteasome alpha 1 subunit |
A4HP20 | 29725.7 | 5.04 | 0 | 10.57 | 2 | |
| Proteasome alpha 7 subunit |
A4H9T4 | 34861.8 | 7.39 | 0 | 7.14 | 2 | |
| Putative | A4H5E1 | 63469.70 | 4.58 | 2 | 3.51 | 2 | |
| Putative | A4HRM8 | 72826.10 | 5.40 | 2 | 3.33 | 2 | |
| Putative | A4HUH4 | 81190.5 | 5.94 | 9 | 2.91 | 2 | |
| Putative | A4I077 | 14107.10 | 7.84 | 0 | 17.80 | 2 | |
| Putative | A4I0D6 | 57285.50 | 7.76 | 3 | 5.88 | 2 | |
| Putative | A4I4S8 | 155409.8 | 5.24 | 10 | 3.48 | 4 | |
| Putative | A4I6U4 | 31523.70 | 8.19 | 0 | 9.93 | 2 | |
| Putative | A4I7J1 | 140540.3 | 5.01 | 6 | 4.22 | 5 | |
| Putative | A4I9W1 | 37830.4 | 7.75 | 3 | 15.34 | 4 | |
| Putative | A4IBR6 | 71206.1 | 6.39 | 5 | 3.84 | 2 | |
| Reiske iron-sulfur protein precursor |
A4HMH5 | 33752.8 | 6.15 | 2 | 8.42 | 2 | |
| Reticulon domain protein | Q4Q737 | 22145.70 | 9.79 | 3 | 14.21 | 2 | |
| Surface Antigen Protein 2# | A4HV45 | 43941.8 | 5.31 | 3 | 5.04 | 2 | |
| Vacuolar ATP synthase catalytic subunit a |
A4HB86 | 67370.9 | 5.06 | 2 | 3.93 | 2 | |
| Vacuolar ATP synthase catalytic subunit a |
A4IAD4 | 67676.0 | 4.97 | 2 | 3.77 | 2 | |
| Vacuolar proton translocating ATPase subunit A |
A4I0M2 | 87674.00 | 4.92 | 7 | 4.26 | 2 | |
| Vesicle-associated membrane protein |
A4H570 | 24223.40 | 8.24 | 1 | 11.63 | 2 |
#
Predicted to have a GPI anchor