Skip to main content
. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Biochem J. 2010 Apr 28;428(1):47–54. doi: 10.1042/BJ20100016

Figure 1. Apolipoprotein E inhibition of LPS and poly(I-C) stimulation of pro-inflammatory cytokine production by RAW 264.7 cells.

Figure 1

Murine RAW 264.7 cells were pre-incubated with apoE for 30 min at the concentrations indicated prior to the addition of 25 ng/ml LPS (panels A–C) or 25 μg/ml poly(I-C) (panels D–F). The incubation was continued at 37 ºC for 8 h and the culture medium was collected. The concentration of IL-6 (panels A, D), IL-1β (panels B, E), and TNF-α (panels C, F) in the culture medium was detected by immunoassay or ELISA kits. The data represent means ±S.D. from 2 separate experiments, each performed in triplicates. * indicates significant difference from samples incubated without apoE at P<0.01.