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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Biochem J. 2010 Apr 28;428(1):47–54. doi: 10.1042/BJ20100016

Figure 3. Apolipoprotein E and apoE(141–155)2 peptide inhibit LPS and poly(I-C) stimulation of JNK and cJun phosphorylation.

Figure 3

Murine RAW 264.7 cells were pre-incubated with or without 50 μg/ml apoE or 0–10 μM of the apoE(141–155)2 peptide prior to the addition of 25 ng/ml LPS or 25 μg/ml poly(I-C). Panel A shows LPS and poly(I-C) induction of JNK phosphorylation in a time-dependent manner. Induction of both JNK1 and JNK2 bands are indicated from the blots. Panel B shows LPS and poly(I-C) induction of c-Jun phosphorylation in a time-dependent manner. Panels C and D show apoE and apoE(141–155)2 peptide, respectively, inhibited LPS induced JNK and c-Jun phosphorylation 60 min after the addition of the TLR agonists. The data are derived from samples pooled from triplicate plates and are representatives of 2 separate experiments.