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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Biochem J. 2010 Apr 28;428(1):47–54. doi: 10.1042/BJ20100016

Figure 4. Reductive methylation abolished apoE inhibition of LPS induced macrophage activation but had no effect on apoE inhibition of poly(I-C)-induced events.

Figure 4

Murine RAW 264.7 macrophages were pre-incubated with 50 μg/ml of apoE or methylated apoE (MeApoE) prior to the addition of 25 ng/ml LPS or 25 μg/ml poly(I-C). Cell lysates were prepared after 60 min to detect JNK and cJun phosphorylation induced by LPS (panel A) or poly(I-C) (panel B). Panel C compares the influence of apoE versus MeApoE on IL-6 production after 8 h stimulation with LPS or poly(I-C). The data are means ± S.D. from triplicate plates in 2 separate experiments. Bars with different letters indicate significant difference at P<0.01.