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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Thromb Haemost. 2011 Mar;9(3):531–539. doi: 10.1111/j.1538-7836.2010.04167.x

Fig. 1.

Fig. 1

Time-dependent inhibition of free FVIIa or FVIIa complexed with relipidated TF by PAI-1. FVIIa (10 nM) (panel A) or FVIIa-TF complexes (FVIIa, 10 nM; relipidated TF, 50 nM) (panel B) were incubated with control buffer (□), heparin (10 U/ml) (△), vitronectin (1 μM) (inverted △), PAI-1 (1 μM) (○), PAI-1 (1 μM) + heparin (10 U/ml (▲), or PAI-1 (1 μM) + vitronectin (1 μM) (●). At different time intervals (0-120 min), aliquots were removed from the reaction mixtures and diluted in TBS/BSA buffer containing 2 mM EDTA. FVIIa coagulant activity was measured in the FVIIa specific clotting assay. FVIIa activity measured at 0 min time point was taken as 100%. Data represent the mean ± SEM (n =3).