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. 2010 Jul 21;95(10):4762–4770. doi: 10.1210/jc.2010-0354

Figure 1.

Figure 1

Western immunoblotting confirming the specificity of polyclonal antibodies to MCT8 and MCT10. A, MCT8 4790 antibody. Whole cell protein extracts of MCT8-null JEG3 cells transfected with either vector only (VO) or a plasmid encoding human MCT8 and homogenates of term placenta tissue (Term PL) expressing endogenous MCT8 were probed with the MCT8 antibody (1.6 μg/ml) after preincubation with or without the blocking peptide (BP). B, MCT10 2198 antibody. Whole cell protein extracts of HTR8/SVNeo cells (with very low endogenous MCT10 mRNA expression) transfected with either VO or a plasmid encoding human MCT10 and extracts from normal cytotrophoblasts (CTs) that were cultured for 18 h were probed with the MCT10 antibody (5.2 μg/ml) after preincubation with or without the blocking peptide (BP). Bands of approximately 60 kDa for MCT8 (A) and approximately 50 kDa for MCT10 (B) are seen, consistent with their predicted molecular weights, respectively. Immunoreactivity for β-actin was used to assess protein loading.