Skip to main content
. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Thromb Haemost. 2011 Mar;9(3):562–573. doi: 10.1111/j.1538-7836.2010.04168.x

Figure 1. Tat induces human platelet activation.

Figure 1

Histogram of flow cytometry analysis of the platelets treated with Tat or Ad18-66 (Ctrl, filled histogram) with CD154 (a) or CD62P (b). Histogram is an example of one of three experiments. Bar graph summary of Tat induced platelet activation and CD154 expression (c). Both Tat and CCL5 induce mouse platelet activation. Histogram of flow cytometry analysis of the platelets treated with Tat, CCL5 or Ad18-66 (Ctrl, filled histogram). Histogram is an example of one of three experiments. Summary of Tat and CCL5 induced mouse platelet activation (f). Tat interacts with platelets through integrin β3. Platelets incubated with S35- Tat or S35-luciferase in PBS for 30 minutes at 37°C, washed and lysed for total protein were resolved on a SDS-PAGE gel (g). Western blot analysis for β3 with GST-Tat or GST pulls down protein from platelet lysates (h). Western blot analysis of CD154 release in supernatant of platelets incubated with Tat or with PBS buffer as control (i).