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Western blot analysis of apoptosis-inducing factor (AIF) and c-Jun-N-terminal Kinase (JNK) in the cytosolic fraction and of Bax, JNK and the activated form phospho-JNK (PJNK) in the mitochondrial fraction from untreated controls and 3 and 6 h after 200 mg/kg APAP in wild type (WT) and SOD2-deficient (SOD+/−) mice. Beta actin was used as a loading control for cytosolic proteins.
