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. Author manuscript; available in PMC: 2012 Jun 30.
Published in final edited form as: Chem Biol Interact. 2010 Oct 8;192(1-2):122–128. doi: 10.1016/j.cbi.2010.09.032

Figure 1. Relative modification of arginine sites by methyglyoxal.

Figure 1

Human serum albumin (75 μM final) was treated with a 2-fold dilution series (0.39–100 μM final) of MG from 100 μM in 1X PBS pH 7.4. MG-modified albumin was reduced, alkylated, digested, and analyzed by LC-MS/MS in MRM mode using the transitions in Table 1. Peak AUC’s were normalized to the unmodified tryptic albumin peptide LVNEVTEFAK.