Figure 3.

Adhesion of the oral keratinocyte cell line H357 and primary normal oral keratinocytes (NOK) to human αE I domain fusion proteins. (a) The percentage of H357 and NOK cells binding to ‘open’, wild-type and ‘closed’ fusion proteins in the presence (+) and absence (−) of Mn²⁺. Background adhesion to bovine serum albumin (BSA) was subtracted. *, P < 0·001; #, P < 0·001; **, P < 0·001; and ##, P < 0·001. (b) The effect of mAbs (HECD-1 and E4.6) to E-cadherin on the binding of H357 and NOK cells to ‘open’ fusion protein in the presence of Mn²⁺. Background adhesion to BSA was subtracted. (c) The percentage of H357 cells bound to ‘open’, wild-type and ‘closed’ fusion proteins in the presence and absence of dithiothreitol (DTT). Background adhesion to BSA was subtracted. All results were combined from three separate experiments. Error bars represent the standard error of the mean (SEM). WT, wild type.