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hrp36 is the only major hnRNP protein that regulates the fidelity of exon 6 mutually exclusive splicing. (a,b) D. melanogaster S2 cells were treated with hrp36, hrp38, hrp40 and hrp48 double-stranded RNAs as indicated. Exon 6 splicing was examined by RT-PCR with primers to detect the specified exons (a), and the extent and specificity of depletion was monitored by western blotting (b). Note that the hrp38 antibody cross-reacts with hrp36 to some extent.
