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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Curr Opin Chem Biol. 2010 Jul 31;14(6):721–727. doi: 10.1016/j.cbpa.2010.07.002

Figure 4.

Figure 4

Activity-based protein profiling (ABPP) as a tool for monitoring inhibitor selectivity. A. Fluorophosphonate chemistry for ABPP of serine hydrolases. B. Protocol for testing the selectivity of a serine hydrolase inhibitor (light green triangle). The figure depicts an ideal situation in which six serine hydrolases (different colored pies) are treated with a tagged fluorophosphonate in the presence or absence of the inhibitor. If the inhibitor is specific for the blue hydrolase, then its signal in the profile will disappear, but that of the other proteins will be unaffected. The figure depicts the use of gel-based analysis with blotting specific for the tag to monitor protein labeling.