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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Curr Opin Chem Biol. 2010 Nov 17;15(1):48–56. doi: 10.1016/j.cbpa.2010.10.021

Figure 3.

Figure 3

Orthogonal Active Site Identification System (OASIS) for proteomic identification and study of PKS and NRPS enzymes. Natural product producer proteomes are enriched with domain-specific probes (RG = protein reactive group), followed by MudPIT MS analysis. The collection of multiple datasets using orthogonal probes allows specific identification of modular biosynthetic enzymes, which contain multiple active sites on a single polypeptide. The application of orthogonal probes can also be used to facilitate study of specific subsets of natural product biosynthetic enzymes.