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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Endocrinol. 2010 Jul 30;207(1):67–75. doi: 10.1677/JOE-10-0181

Figure 4. Response of Hyp to FGF23 delivery.

Figure 4

Results from vehicle-injected Hyp mice are shown in the upper panels, and FGF23-injected Hyp mice in the lower panels at 10 min post-injection. Phospho-ERK1/2 staining was only observed in the FGF23-injected animals; Klotho (KL) was positive in both vehicle- and FGF23-injected animals. p-ERK1/2 staining (red) localized to the nucleus in the same nephron segment as KL (green) in FGF23-injected mice as shown by p-ERK1/2 and KL co-staining (‘Merge’ column; arrows show positive nuclear p-ERK1/2 co-localized with KL). Nuclei were stained blue using DAPI in the Merge column.