Skip to main content
. 2011 Feb 16;31(7):2615–2623. doi: 10.1523/JNEUROSCI.4767-10.2011

Figure 3.

Figure 3.

Downregulation of PS1 in neurosphere cultures enhances their differentiation without affecting multipotentiality. A, Neurosphere culture transduced with lentiviral vectors expressing PS1 1.1, PS1 4.11, IR 8.1, IR 9.1 express GFP (left and right panel), as well as the neural progenitor cell marker nestin (right panel; orange; nestin+GFP+; blue, DAPI). B, Neurospheres transduced with lentiviral vectors expressing PS1 1.1, PS1 4.11 for PS1 targeting readily differentiate, while neurospheres transduced with lentiviral vectors expressing IR 8.1 or IR 9.1, or GFP-Lenti control maintain sphere morphology (green, GFP; red, β-tubulin; blue, DAPI). C, Singly dissociated neural progenitor cells transduced with lentiviral vectors expressing PS1 1.1, PS1 4.11 for PS1 targeting exhibit enhanced differentiation compared with progenitors transduced with lentiviral vectors expressing IR 8.1 or IR 9.1 control (green, GFP; red, β-tubulin; blue, DAPI). D, Number of neurospheres (left panel) generated from neural progenitor cells infected with lentiviral vectors expressing PS1 1.1, PS1 4.11, is significantly reduced, while the number of differentiated cells is increased (right panel) compared with neural progenitor cells infected with lentiviral vectors expressing IR 8.1, IR 9.1 siRNA, or GFP-Lenti. E, Neural progenitor cells infected with lentiviral vectors expressing PS1 1.1, PS1 4.11 can give rise to neurons (GFP+β-tubulin+), astrocytes (GFP+GFAP+) and oligodendrocytes (GFP+PDGFR+), suggesting that downregulation of PS1 does not affect their multipotentiality. F, Neural progenitor cells infected with lentiviral vectors expressing PS1 1.1, PS1 4.11 give rise to increased number of differentiated cells of all three lineages, neurons, astrocytes and oligodendrocytes. t test *p < 0.05.