Figure 4.
PS1 effect on neural progenitor cells is γ-secretase-dependent. A, B, Neural progenitor cells treated with the γ-secretase inhibitor L-685,458, either as neurospheres (A) or as singly dissociated cells (B) differentiate faster than progenitors treated with vehicle (green, GFAP; red, β-tubulin; blue, DAPI). C, Quantification of the length of cell processes reveals significantly more processes in neural progenitor cells treated with L-685,458 compared with vehicle. D, Quantitative analysis of cell lineage reveals a slight increase in the number of new neurons and astrocytes in cells treated with L-685,458 or DAPT and a significant increase in the number of new oligodendrocytes. E, Neural progenitor cells treated with L-685,458 or DAPT differentiate into β-tubulin+ neurons, S100β+ astrocytes and PDGFR+ oligodendrocytes. t test *p < 0.05.