Figure 5.

Reduced PS1 expression impairs proliferation and affects clone formation capacity. A, Neurospheres infected with lentiviral vectors expressing siRNA for PS1 targeting exhibit reduced uptake of the thymidine analog BrdU compared with neurospheres infected with an irrelevant IR 8.1, 9.1 siRNA. B, A clonogenic assay shows that the number of clone forming cells in NPCs infected with lentiviral vectors expressing siRNA for PS1 targeting is significantly reduced compared with NPCs infected with an irrelevant IR 8.1, 9.1 siRNA. C, D, Neurospheres treated with L-685,458 (C) or DAPT (D) exhibit reduced uptake of BrdU compared with neurospheres treated with vehicle. t test *p < 0.05.