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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Cell Physiol. 2011 May;226(5):1383–1389. doi: 10.1002/jcp.22465

Fig. 1. RUNX2 remains bound on mitotic chromosomes throughout mitosis.

Fig. 1

U2OS human osteosarcoma cells were transfected with EGFP-RUNX2 and mRFP-Histone H2B. Mitotic cells were located using mRFP-Histone H2B and imaged through mitosis. RUNX2 punctate foci as well as diffuse RUNX2 staining on chromosomes were clearly evident in prophase, metaphase, anaphase and telophase. Left column—EGFP-RUNX2; middle column—mRFP-H2B and DIC in insert for comparison; right column—the merged image. Size bar is 10 μm.