Fig. 2. RUNX2 binding kinetics in interphase non-nucleolar foci.
In interphase cells, RUNX2 subnuclear foci, sites of RNA polymerase II transcription, were photobleached for 2 seconds and recovery was monitored for 80–120 seconds. The Differential Interference Contrast (DIC) image is shown for comparison. The calculated half-time (t1/2) of recovery was 12 seconds and the immobile fraction was 35%. Size bar is 10 μm.
