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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Cell Physiol. 2011 May;226(5):1383–1389. doi: 10.1002/jcp.22465

Fig. 4. Photobleach recovery of RUNX2 on chromosomes during metaphase.

Fig. 4

Multiple metaphase cells expressing EGFP-RUNX2 and mRFP-Histone H2B were used for FRAP analysis. (A) The images show the region of interest in cells before photobleaching, just after photobleaching, and 80 seconds later when the fluorescence had recovered. The size bar is 10 μm. (B) Normalized mean fluorescence recovery curves over time were calculated for chromosome bound RUNX2; the means were plotted with standard errors for each time point. Cells in metaphase show a t½ of recovery of 4 seconds and the immobile fraction (41%) had decreased since prophase.