Skip to main content
. 2011 Apr 1;138(7):1409–1419. doi: 10.1242/dev.060046

Fig. 4.

Fig. 4.

Heart field migration is sensitive to Robo1 dosage. (A) Endocardial migration was defective at 20 somites in zebrafish embryos injected with 75 pg Robo1 mRNA (mCherry mRNA was utilized as a control). (B) Rescue of migration defects in miR-218 morphants was accomplished by co-injecting sub-phenotypic doses of robo1 MO. Quantification of migration defects is shown beneath. *, P<0.05 (Fisher's exact test for a 2×3 contingency table). (C) Analysis of endocardial migration at the 20-somite stage in Tg(hsp70l:slit2-GFP) embryos that were heat shocked at 5 somites. Embryos lacking the transgene were used as a control. Disorganization of the endocardium was evident in Slit2-overexpressing embryos. Scale bars: 50 μm.