Figure 3.
Experimental assessment of the time-course changes in stability of solubilized Cyt bo3 and activity of LeuT WT. (a) CPM assay for Cyt bo3 was performed at 40°C for 130 minutes using CMC + 0.04 wt % amphiphile. LeuT WT was kept at room termperature up to 12 days in the presence of CMC + 0.04 wt % (b) or CMC + 0.2 wt % (c) before determining binding activity by SPA.