Figure 1.
Structures of pGAD10, pGadXY, pGadX, and pGadY. pGAD10 was the vector used to clone gadXY, gadX, and gadY. pGadXY has a 1,470-bp fragment containing gadX, gadY, and a portion of gadW of E. coli K-12 genomic DNA inserted into the EcoRI site of pGAD10. pGadX contains a DNA fragment carrying the 825-bp gadX also inserted into the EcoRI site of pGAD10. pGadY is derived from pGadXY by deleting the 601-bp NcoI-DraIII fragment and thus contains a truncated gadX, the entire gadY, and a portion of gadW. Nucleotide sequences of the promoter regions of gadX and gadY are shown. The orientation of gadX is opposite to that of gadY. The sigma factor S (RpoS) recognition site and the Shine-Dalgarno (SD) sequence are shown in the 5' end region of gadX. PADH is the promoter of GAL4-AD and is not functional in E. coli.