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. 2011 Feb 11;11:33. doi: 10.1186/1471-2180-11-33

Table 5.

Oligonucleotide primers used in this study

Primer Sequence 5 '- 3'
F/cea7-BamHI GGATCCATGAGCGGTGGAGATGGACG
R/cei7-PstI CTGCAGTCAGCCCTGTTTAAATCC
F/btuB-219-XbaI GGCTCTAGAAAACGGTGCCATCATACTTTG
R/btuB+242-HindIII GGCAAGCTTATCATTGTAAAGCATCCACAATAG
F/btuB-767 GTTCACCGTTGCTCGATACC
R/btuB-1087 TCAGATAGATGCCGGTATTACG
F/btuB-431-XbaI GCTCTAGAACGGGATTATTACGC
F/btuB-671-XbaI GCTCTAGATCATCTCTTTCCC
F/btuB-1043-XbaI GCTCTAGACCGCTGCGCGGA
R/lacZ TTATTTTTGACACCAGACC
F/gadA-176 GATCGCCCGAACAGCAA
R/gadA+77 CGTGAATCGAGTAGTTC
F/gadB-173 AATAACAGCATAAAACA
R/gadB+77 CGTGAATCGAGTAGTTCC
F/pal-XbaI TCTAGAGAGGCGTACAAGTTCTG
R/pal-HindIII AAGCTTATCATTTCAATGATTCCTTTAC
F/gadX-BamHI GGATCCATGCAACCACTACATGG
R/gadX-PstI CTGCAGCTATAATCTTATTCCTT
F/gadX-393 TATACCGCTGCTTCTGAACG
R/gadX-726 TCGCTCCTGATACTCTGTGG
F/rrsA-483 CGTTACCCGCAGAAGAAGC
R/rrsA-808 GTGGACTACCAGGGTATCTAATCC

The underlined letters indicate restriction sites.