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SDS-PAGE Analysis of total membrane proteins. N. europaea wild type and fur:kanP mutant in Fe-replete (10 μM) (lanes 1, 3) and Fe-limited (0.2 μM) media (lanes 2, 4). Over-expression of proteins with molecular weights similar to outer membrane Fe-siderophore receptors indicated by * was observed in fur:kanP mutant in both Fe-replete and Fe-limited media.
