Table 3.
Codon usage in mce1 and mce4 operons
| Operon | Gene name (Accession Number) | Wild type codon | Polymorphic codon |
|---|---|---|---|
| mce1 operon |
mce1A [Rv0169] |
TAC | TAT |
|
yrbE4A [Rv3501c] |
GCG ATC |
GCT ATA |
|
| mce4 |
yrbE4B [Rv3500c] |
ATC CCC |
ATT CCT |
| operon |
mce4A [Rv3499c] |
TTC | TTT |
|
lprN [Rv3495c] |
GCC | GCT | |
|
mce4F [Rv3494c] |
ACC | ACA | |
The codon usage in the polymorphic regions is shown here. The synonymous changes in the nucleotide sequence, when analysed bioinformatically through Gene Runner software version 3.05 (Hastings Software, Inc.) predicts the usage of less preferred codon which could reflect upon the expression efficiency of the protein encoded by the gene.
Nucleotide highlighted in bold indicates the altered nucleotide.