Table 1.
Evaluation of quality and quantity parameters of RNA samples extracted from SK-N-MC neuroblastoma.
| Kit | A230 | A260 | A280 | A260/A280 | A260/A230 | Concentration (ng/μL) | Elution volume (μL) | Yield (μg RNA/1E6 cells) |
|---|---|---|---|---|---|---|---|---|
| AxyPrep Multisource Total RNA Miniprep Kit (Axygen) | 5.38 | 6.82 | 3.29 | 2.07 | 1.26 | 272.80 ± 28.55 | 100 | 3.94 ± 0.41 |
| RNeasy® Mini Kit (Qiagen) | 1.32 | 2.14 | 1.03 | 2.07 | 1.63 | 85.74 ± 72.26 | 50 | 0.62 ± 0.52 |
| EasySpin (Citomed) | 1.79 | 3.54 | 1.73 | 2.06 | 1.92 | 141.5 ± 74.58 | 50 | 1.02 ± 0.54 |
| Illustra RNAspin Mini RNA Isolation Kit (GE) | 0.3 | 0.15 | 0.07 | 2.13 | 0.37 | 5.98 ± 7.44 | 100 | 0.09 ± 0.11 |
| TRIzol® Plus RNA Purification System (Invitrogen) | 1.13 | 2.31 | 1.15 | 1.86 | 1.65 | 92.4 ± 111.50 | 50 | 0.67 ± 0.80 |
| E.Z.N.A. ™ Total RNA Kit II (Omega bio-tek) | 0.30 | 0.55 | 0.29 | 1.89 | 1.52 | 21.87 ± 4.14 | 50 | 0.16 ± 0.03 |