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. 2011 Feb 18;11:18. doi: 10.1186/1471-2431-11-18

Figure 2.

Figure 2

Experimental approach for performance of HIV-1 gag and pol FP-DBS PCR assay for detection of HIV-1 infection from FP-DBS obtained from HIV-1 positive infants as defined by Roche Amplicor v1.5 assay. 186 recently collected FP-DBS that were determined in the prior ~month to be HIV-1 positive by Roche Amplicor v1.5 assay were tested by HIV-1 gag and pol PCR assays. Of these 178 FP-DBS from the infants were identified as HIV-1 positive. Retesting of subsequent blood samples from the 8 discordant infants showed that they were HIV-1 negative. Thus, the sensitivity of HIV-1 pol PCR assay was found to be 100% (178 of 178) for detection of confirmed HIV-1 positive infants from FP-DBS.